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ms2 stem loops  (Addgene inc)


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    Addgene inc ms2 stem loops
    (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of <t>MS2-MCP-GFP-PXN</t> system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
    Ms2 Stem Loops, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ms2 stem loops/product/Addgene inc
    Average 93 stars, based on 4 article reviews
    ms2 stem loops - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration"

    Article Title: G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.115237

    (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of MS2-MCP-GFP-PXN system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
    Figure Legend Snippet: (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of MS2-MCP-GFP-PXN system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .

    Techniques Used: Control, shRNA, Expressing


    Figure Legend Snippet:

    Techniques Used: Control, Recombinant, SYBR Green Assay, Cell Culture, Protease Inhibitor, Mutagenesis, Isolation, Sequencing, shRNA, Plasmid Preparation, Software



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    (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of <t>MS2-MCP-GFP-PXN</t> system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
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    (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of <t>MS2-MCP-GFP-PXN</t> system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
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    (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of <t>MS2-MCP-GFP-PXN</t> system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .
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    (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of MS2-MCP-GFP-PXN system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .

    Journal: Cell reports

    Article Title: G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration

    doi: 10.1016/j.celrep.2025.115237

    Figure Lengend Snippet: (A) Schematic of G3BP1 protein domains and mutants used. (B and C) Rose plots (B) and quantification of speed (μm/min), represented as boxplots, (C) from individually migrating cells from control or G3BP1 shRNA-treated cells with either no G3BP1, full-length (FL), ΔRBD, or ΔNTF2L re-expression (one-way ANOVA with multiple comparisons, p < 0.0001 for Con or FL vs. shRNA alone, ΔRBD, or ΔNTF2L, and p = 0.0445 for ΔRBD vs. ΔNTF2L; n = 247–346 cells per group). (D) Boxplots of FA-G3BP1 correlation coefficient from control or re-expression cells in E (one-way ANOVA with multiple comparisons, p < 0.0001 for G3BP1 shRNA only vs. all other comparisons, for Con shRNA vs. ΔRBD or ΔNTF2L, or FL vs. ΔNTF2L; p = 0.0115 for FL vs. ΔRBD; n = 15 for all groups, but n = 7 for G3BP1 shRNA alone). (E) Representative images of G3BP1 (yellow) and PXN (magenta) in control or G3BP1 shRNA-treated cells with G3BP1 re-expression. Dotted lines represent outlines of proteins. Dotted boxes in the whole-cell image represent the region highly magnified along the bottom below. (F) Schematic of MS2-MCP-GFP-PXN system. TRAK2-MS2-tagged mRNA was co-expressed with an MCP-GFP-PXN fusion protein to directly link RNA to FAs. (G) Representative images of G3BP1 RNP complexes (yellow) and TRAK2-MS2 (cyan) in HDFs co-expressing MCP-GFP-PXN, which is incorporated into the FAs (magenta). Dotted lines represent outlines of protein/mRNA. Dotted boxes in images represent the highly magnified regions below. (H) Quantification of smFISH images taken of cells expressing MCP-GFP-PXN and either MS2 alone or TRAK2-MS2. Boxplots represent the log2 of FA enrichment for mRNA foci directly on top of FA (co-localization) (two-way unpaired t test, p < 0.0001, n = 21 cells). (I) Boxplots of FA-G3BP1 correlation coefficient from MS2-only- or TRAK2-MS2-expressing cells (two-way unpaired t test, p = 0.0472, n = 25 cells). (J and K) Rose plots (microns) (J) and quantification of speed (μm/min), represented as boxplots, (K) from individually migrating cells from control or G3BP1 shRNA-treated cells with either MS2 only or TRAK2-MS2 (one-way ANOVA with multiple comparisons, p < 0.0001 for MS2 only vs. TRAK2-MS2 Con shRNA and MS2 only or TRAK2-MS2 Con shRNA vs. both G3BP1 shRNA groups; n = 498–755 cells/group). See also and , , , , , , , , and .

    Article Snippet: MS2 Stem loops were cloned from pET263-pUC57 24xMS2V7 (Addgene, #140705).

    Techniques: Control, shRNA, Expressing

    Journal: Cell reports

    Article Title: G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration

    doi: 10.1016/j.celrep.2025.115237

    Figure Lengend Snippet:

    Article Snippet: MS2 Stem loops were cloned from pET263-pUC57 24xMS2V7 (Addgene, #140705).

    Techniques: Control, Recombinant, SYBR Green Assay, Cell Culture, Protease Inhibitor, Mutagenesis, Isolation, Sequencing, shRNA, Plasmid Preparation, Software